Figure 4

Cytokines  produced by Teff cells after DP stimulation in absence or presence of Treg cells. Box plots represent comparative analysis of percentages of Teff cells producing IL-10 (CD3⁺CD4⁺IL-10⁺) (A), IL-4 (CD3⁺CD4⁺IL-4⁺) (B) and IL-9 (CD3⁺CD4⁺IL-9⁺) (C) from treated (N = 16) and IL-10 (CD3⁺CD4⁺IL-10⁺) (D), IL-4 (CD3⁺CD4⁺IL-4⁺) (E) and IL-9 (CD3⁺CD4⁺IL-9⁺) (F) from untreated (N = 8) patients in different combinations of Teff and Treg at 0 or 12 months (0 M and 12 M, respectively). Statistical analysis was carried out by Wilcoxon test; p-values ≤ 0.05 are considered statistically significant.