Figure 1
The spatiotemporal relationship between Ntn1 expression and CA growth in the developing mouse hindbrain. (a–c) X-gal staining in whole-mount preparations of E10.5 (a), E11.5 (b) and E12.5 (c) Ntn1 +/LacZ mouse hindbrains (E10.5, n = 12; E11.5, n = 8; E12.5, n = 8). The Ntn1 +/LacZ mouse harbors a ß-geo gene trap vector13, allowing Ntn1 expressions to depict by X-gal histochemistry. X-gal reaction products are found in the approximately ventral two-thirds of the hindbrain, in addition to the FP. Dorsal is upwards and rostral is towards the left. (d–g) X-gal histochemistry (blue) and Robo3 immunostaining (brown) in E9.5 (d), E10.5 (e) E11.5 (f) and E12.5 (g) Ntn1 +/LacZ mouse hindbrain transverse sections at the rhombomere 7/8 level (E9.5, n = 4, E10.5, n = 4; E11.5, n = 3; E12.5, n = 3). At all these stages, X-gal products are detected in the FP and the ventral two-thirds of VZ along the entire or most part of the circumferential path of Robo3+ axons. Note that X-gal reaction products do not necessarily reflect Ntn1 protein localization. CP, cerebellar primodium; TG, trigeminal ganglia. The bar in (a) and (g) apply to (a–c) and (d–g), respectively.
