Figure 6
RCN-3 blocked collagen induction by several profibrotic stimulus. Effect of recombinant RCN-3 (0.1 µg/mL) on collagen type I (A) and collagen type III (B) protein levels induced by Aldo (10−8M, Gal-3 (10−8M) and CT-1 (10−7M) in human cardiac fibroblasts. Dose-response of angiotensin II (Ang II; 10−9M-10−7M) on RCN-3 (C). Effects of RCN-3 (0.1 µg/mL) on collagen type I (D) and collagen type III (E) protein levels induced by Ang II (10−7M) in human cardiac fibroblasts. Histogram bars represent the mean ± SEM (n > 6 wells at 70% of confluence per condition) of the three independent experiments. Densitometry values were normalized to stain free gel. *p < 0.05; **p < 0.01; ***p < 0.001 vs. Control. $p < 0.05 vs. Aldo. #p < 0.05 vs. Gal-3. &p < 0.05 vs. CT-1. ††p < 0.01; †††p < 0.001 vs. Ang II.
