Table 6 The PCR mixture system.

From: Ultrasensitive Nano-rt-iPCR for Determination of Polybrominated Diphenyl Ethers in Natural Samples

Reagents

50 μL reaction mixture system

SGExcel FastSYBR Mixture

25 μL

Forward Primer, 5 µM

5 μL

Reverse Primer, 5 µM

5 μL

RNase-Free ddH2O

up to 50 μL