Table 6 The PCR mixture system.
From: Ultrasensitive Nano-rt-iPCR for Determination of Polybrominated Diphenyl Ethers in Natural Samples
Reagents | 50 μL reaction mixture system |
|---|---|
SGExcel FastSYBR Mixture | 25 μL |
Forward Primer, 5 µM | 5 μL |
Reverse Primer, 5 µM | 5 μL |
RNase-Free ddH2O | up to 50 μL |