Figure 4
From: RORα2 requires LSD1 to enhance tumor progression in breast cancer
RORα2 increases cell motility and migration in breast cancer cells. (A) Photomicrographs from the scratch-motility assay of MCF7 (upper panel) and MDA-MB-231 (bottom panel) cells expressing shRNA against RORα2. MCF7 and MDA-MB-231 cells were wounded with the micro-pipette tip. Wound closure was monitored by photomicrography every 24 hr intervals for 48 hrs. Cell migration (%) was quantified by calculating the wound width as shown in the right panel graph. P value is shown from Student’s t test analysis. Error bars represent S.E.M. *p < 0.005. (B–D) Photomicrographs (100X) from transwell migration assay of MCF7 cells (B) and MDA-MB-231 cells (C) expressing shRNA against RORα2 or MCF7 cells expressing shRNA against RORα2 WT or RORα2 E542K (D) for 16 hrs. The bar in photomicrographs represents 100 μm. Graphs show the mean number of migrated cells per field, and p value is shown from Student’s t test analysis. Error bars represent S.E.M. *p < 0.005; **p < 0.001.
