Figure 6 | Scientific Reports

Figure 6

From: Cell-free synthesis of functional antibodies using a coupled in vitro transcription-translation system based on CHO cell lysates

Figure 6

Steady-state affinity analysis of CECF-produced IgG. (a) Scheme depicting the applied work flow: Sample preparation included cell-free synthesis of IgG in CECF reactions and release of translocated proteins from the lumen of microsomal vesicles, followed by subsequent immobilization of antibodies to Protein A coated sensor chip. (b) Overlay of Biacore sensorgrams from two consecutive experiments showing the binding of peptide antigen SMAD2-P to immobilized IgG. Peptide antigen SMAD2-P was injected with increasing concentrations to determine the affinity in steady-state. Sensorgrams are reference subtracted (HC). (c) Binding curve derived from b confirming the functional activity of cell-free produced IgG with a KD of 1.7 µM.

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