Figure 2
P-SHG image analysis from different tissues. (a) rat EDL tendon, (b) rat skin and (c) rat EDL muscle. The first row represents typical 512 × 512 pixels SHG images obtained by averaging the polarization stack. Note that these images correspond to the square root of the SHG intensities. The second row is the angular fibrillar orientation map γ for each pixel of the SHG images. The scale is in degrees and is indicated by the right vertical color bar. Note that direction for 0° is north. The third row corresponds to the 2D distribution of ρ exp for each pixel of the SHG images. The last row represents the distribution of ρ exp (red dots) and ρ poiss (blue dots) as a function of the P-SHG stack mean photons number per pixel Nph for the ROI indicated in the SHG images (first row). Their corresponding mean values are full lines in respectively red and blue colors. Theoretical mean values \({\rho }_{th}\) without Poisson noise are indicated by the horizontal white dotted lines. The best correlation coefficient rk between \({\rho }_{\exp }\) and ρ poiss is obtained for values of \({\theta }_{H}\), \({\theta }_{3H}\), \({\theta }_{SC}\) and \({\theta }_{T}\) angles that are written in the inset of each figure. Scale bars are 10 μm.
