Figure 3 | Scientific Reports

Figure 3

From: Determination of extracellular matrix collagen fibril architectures and pathological remodeling by polarization dependent second harmonic microscopy

Figure 3The alternative text for this image may have been generated using AI.

Classification of different collagen rich tissues based on P-SHG analysis. The first class corresponds to (a) rat EDL epimysium and (b) rat tracheal perichondrium. This class is characterized by preponderant two helical levels of organization \({\theta }_{H},{\theta }_{3H}\) found in straight fibrils. Note that for rat tracheal perichondrium, constant tilt \({\theta }_{Tc}\) has been added in the simulation to better-fit experimental data (see Fig. 1e). This is justified by the fact that undulations are clearly visible on the SHG image. The second class includes (c) rat aorta media, (d) rat aorta adventitia and (e) rat EDL perimysium. It is characterized by three helical levels of organization \({\theta }_{H},{\theta }_{3H},{\theta }_{SC}\). The third class encompasses (f) rat aorta intima, (g) liver vessel, (h) mousse heart epimysium, (i) rat femoral cortex bone (longitudinal section) and (j) mouse femoral cortex bone (transverse section). This latter class can be differentiated from the formers by significant disorder of collagen fibrils necessitating an additional level of fibrillar disorder \({\theta }_{Tr}\). Color-coded plots of the last row have the same meaning as in Fig. 2. Scale bars are 20 μm.

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