Figure 4 | Scientific Reports

Figure 4

From: Determination of extracellular matrix collagen fibril architectures and pathological remodeling by polarization dependent second harmonic microscopy

Figure 4The alternative text for this image may have been generated using AI.

SHG and fluorescence images analysis of control and fibrotic mouse livers. (A) SHG (average of polarization stacks images), autofluorescent and merge images of control and CCl4 treated mice livers taken at lower magnification (10X). Note the presence of vascular (arrowheads) and granular (arrows) collagen in both control and CCl4 treated livers (see merge). Note also that fibrillar collagen between vessels is a characteristic feature of fibrotic livers of CCl4 treated mice. Scale bars are 200 μm. (B) P-SHG image analysis of collagen fibrils organization in control and fibrotic mouse liver vessels taken at higher magnification (60X). First and second rows represent respectively control and CCl4 treated mice vessels. Column (a) represents typical SHG images of control and CCl4 treated mice vessels. Column (b) is the merge of autofluorescent and SHG images. Column (c) is the pixel-resolved angular orientation γ of fibrils in degrees indicated by the bottom horizontal color bar. Note that direction for 0° is north. Column (d) corresponds to the 2D distribution of ρ exp for each pixel of the SHG images. Column (e) represents the distribution of ρ exp and ρ poiss and their means as a function of the P-SHG stack mean photons number per pixel Nph for the ROI indicated in the SHG image (first column). Color-coded plots have the same meaning as in Fig. 2. Scale bars are 20 μm.

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