Figure 2
From: Response of human periodontal ligament stem cells to IFN-γ and TLR-agonists
Effect of IFN-γ, Pam3CSK4, and E. coli LPS on intracellular expression of IDO-1 in human periodontal ligament stem cells. Cells were stimulated with IFN-γ (10 ng/ml), TLR2 agonist Pam3CSK4 (1 µg/ml), TLR4 agonist E. coli LPS (1 µg/ml), or combinations of IFN-γ and TLR agonists for 48 h. After stimulation, cells were stained intracellulary with anti IDO-1 antibody and proceeded to flow cytometry analysis. (A) original FACS dot plots of hPDLSCs stained intracellularly with anti-human IDO-1 antibody after stimulation with different stimuli. (B) percentage of hPDLSCs stained positively with anti-human IDO-1 antibody. (C) mean fluorescence intensity of hPDLSCs positively stained with anti-human IDO-1 antibody. Data are presented as mean ± s.e.m. of 6 different donors. *significantly different compared to control, p < 0.05. #significantly different between groups, p < 0.05.
