Figure 1 | Scientific Reports

Figure 1

From: Up-regulation of claudin-2 expression by aldosterone in colonic epithelial cells of mice fed with NaCl-depleted diets

Figure 1

Effect of NaCl depletion on colonic claudins expression and serum ALD concentration. (A) The distal colon was isolated from the mice fed normal and NaCl-depleted diets. Cytoplasmic extracts including membrane and cytoplasmic proteins of colon were prepared from three independent preparations and immunoblotted with anti-claudin-2, -4, -7, -8, -15, MR, GR, or β-actin antibody. (B) The contents of claudins were represented relative to the values of β-actin. (C) The contents of MR and GR were represented relative to the values of β-actin. (D) Serum ALD concentrations in the mice fed normal and NaCl-depleted diets were measured. n = 3. Statistical comparison was made by t test. **P < 0.01 and *P < 0.05 significantly different from normal. NS, not significantly different.

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