Figure 8

Relative images of immunohistochemical localization of claudin 2 in the mouse colon. The segments of mouse middle and distal colon were isolated from the mice fed normal (n = 3) and NaCl-depleted diets (n = 4). They were stained with claudin-2 (green) and shown on the upper half. Merged images with DAPI staining of the nuclei are shown on the lower half. S and C represent surface and crypt, respectively. The scale bar represents 10 μm.