Figure 5 | Scientific Reports

Figure 5

From: Resveratrol Increases Hepatic SHBG Expression through Human Constitutive Androstane Receptor: a new Contribution to the French Paradox

Figure 5

Resveratrol increases SHBG production through hCAR binding to the DR-1 motif present in the human SHBG proximal promoter. (a) Analysis of SHBG mRNA expression in hCAR-transfected HepG2 cells treated with vehicle or resveratrol (25 µM) during 2 and 6 hours. Human 18 S (h18S) rRNA was amplified as a control. Data points are shown as mean ± SEM of triplicates. *p < 0.05 when compared with the control. (b) ChIP assay of CAR binding to the human GAPDH and SHBG promoters in vehicle and resveratrol treated hCAR-transfected cells. Nonspecific rabbit IgGs were used in ChIP reactions to control for nonspecific immunoprecipitation. Positive PCR controls of sheared genomic DNA templates indicated the integrity of the input DNA used in the ChIP reactions. ChIP results were quantified by real-time PCR. (c) The activities of a wild-type or mutated (to disrupt the consensus DR-1 sequence at 70 bp from the start site of transcription) human SHBG promoter-driven luciferase reporter plasmids were analyzed in HepG2 cells in the presence of hCAR or mCAR expression vectors after treatment with vehicle (control) or resveratrol (25 µM). Data points are mean ± SEM of triplicate measurements. **P < 0.01 compared with the control.

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