Figure 4
From: Identification and characterisation of lamprey protein kinase C delta-like gene
Purification of recombinant PKC-δ-like protein and preparation of polyclonal antibodies. (a) Expression and purification of L. japonica PKC-δ-like recombinant protein. The PKC-δ-like recombinant protein was expressed in E. coli BL21 and analyzed through SDS-PAGE. M, protein marker; lane 1, total protein of uninduced cells; lane 2, total protein of induced cells containing pET-28a-PKC-δ-like; lane 3, purified PKC-δ-like recombinant protein. (b) Indirect ELISA of PKC-δ-like IgG with PKC-δ-like recombinant protein. Serially diluted (1:10,000–1:540,000) polyclonal antibodies were tested against PKC-δ-like recombinant protein using ELISA. Pre-immune IgG was used as a negative control (n = 3). Error bars indicate the standard error of the mean. (c) Western blot analysis of the specificity of rabbit anti-PKC-δ-like polyclonal antibodies. Lane 1, PKC-δ-like recombinant protein; lane 2, supraneural body from L. japonica. Full-length blots are presented in Supplementary Fig. S1.
