Figure 2

Staining of integrin αv, pFAK(Y397) and paxillin on micropillars with heights 4 µm and stiffness 1.014 nN/µm (A1 B1 C1), 8 µm and 0.137 nN/µm (A2 B2 C2) and 18 µm and 0.012 nN/µm (A3 B3 C3). The expression areas and aspect ratio of integrin αv (A4–A5), pFAK(Y397) (B4–B5) and paxillin (C4–C5) were quantified by Image J. (n = 10) The elastic modulus for all micropillars was 30 kPa. Scale bar 20 µm.