Figure 5 | Scientific Reports

Figure 5

From: Preferential sensing and response to microenvironment stiffness of human dermal fibroblast cultured on protein micropatterns fabricated by 3D multiphoton biofabrication

Figure 5

Immunofluorescence staining of integrin αv, pFAK(Y397) and paxillin on micropillars with heights 14 µm and elastic modulus 15 kPa (A1 B1 C1), 16 µm and 30 kPa (A2 B2 C2) and 20 µm and 45 kPa (A3 B3 C3). The expression areas and aspect ratio of integrin αv (A4–A5), pFAK(Y397) (B4–B5) and paxillin (C4–C5) were quantified by Image J. (n = 10) The stiffness and contact area for all micropillars was 0.017 nN/µm and 3.14 μm2. Scale bar 20 µm.

Back to article page