Figure 6 | Scientific Reports

Figure 6

From: Preferential sensing and response to microenvironment stiffness of human dermal fibroblast cultured on protein micropatterns fabricated by 3D multiphoton biofabrication

Figure 6The alt text for this image may have been generated using AI.

Staining of integrin αv, pFAK(Y397) and paxillin on micropillars with heights 7 µm and elastic modulus 15 kPa (A1 B1 C1 D1 E1 F1), 8 µm and 30 kPa (A2 B2 C2 D2 E2 F2) and 10 µm and 45 kPa (A3 B3 C3 D3 E3 F3). The diameter and contact area of micropillars were 4 µm and 12.56 μm2(A–C) and 6 µm and 28.26 μm2 (D–F) respectively. The expression areas and aspect ratio of integrin αv (A4–A5 D4–D5), pFAK(Y397) (B4–B5 E4–E5) and paxillin (C4–C5 F4–F5) were quantified by Image J. (n = 10) Scale bar 20 µm.

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