Figure 6 | Scientific Reports

Figure 6

From: Preferential sensing and response to microenvironment stiffness of human dermal fibroblast cultured on protein micropatterns fabricated by 3D multiphoton biofabrication

Figure 6

Staining of integrin αv, pFAK(Y397) and paxillin on micropillars with heights 7 µm and elastic modulus 15 kPa (A1 B1 C1 D1 E1 F1), 8 µm and 30 kPa (A2 B2 C2 D2 E2 F2) and 10 µm and 45 kPa (A3 B3 C3 D3 E3 F3). The diameter and contact area of micropillars were 4 µm and 12.56 μm2(A–C) and 6 µm and 28.26 μm2 (D–F) respectively. The expression areas and aspect ratio of integrin αv (A4–A5 D4–D5), pFAK(Y397) (B4–B5 E4–E5) and paxillin (C4–C5 F4–F5) were quantified by Image J. (n = 10) Scale bar 20 µm.

Back to article page