Figure 2

EBr induced nucleosome destabilization. (A) EBr induced elution of H2B-GFP and H3-GFP performed on ice and at room temperature (RT) in histone-GFP expressor HeLa nuclei. EBr was applied in the presence of 0.75 M NaCl. (B) Effect of co-treatment with EBr and salt in histone-GFP expressor HeLa nuclei. The shift of elution curves in the presence of 100 µg/ml EBr is seen comparing the continuous and dashed lines. The blue double arrow shows that the maximal effect of EBr on nucleosome stability occurs at 0.75 M salt. (C) EBr elution profiles of H3K4me3 or H3K27me3 in HeLa nuclei. (See also: Supplementary Fig. S1F). (D) EBr elution profiles of H3K4me3 or H3K27me3 in mES and NPC nuclei. (See also: Supplementary Fig. S2). The concentration of the intercalators are shown in a logarithmic scale in panels A, C and D. The elution curves refer to G1 phase cells gated according to their DNA fluorescence intensity distribution and the error bars represent SEM of ~600 G1 nuclei measured by LSC. The cell-to-cell and sample-to-sample C.V. values of the elution profiles are shown in Supplementary Table S1.