Figure 3

Salt induced nucleosome destabilization. (A) Salt elution profiles of antibody detected H2A, of H2B-GFP, H3-GFP and H4-GFP in histone-GFP expressor HeLa nuclei. (B) Salt elution profiles of γH2A.X, H2A.X and H2A, measured in parallel, in the nuclei of HCT116 cells exposed to 25 μM etoposide. (See also: Supplementary Fig. S6). (C) Salt elution profiles of H3K4me3 and H3K27me3 in HeLa nuclei. (See also: Supplementary Fig. S7). (D) Salt elution profiles of H3K4me3 and H3K27me3 in mES and NPC nuclei (See also: Supplementary Fig. S2). The elution curves refer to G1 phase cells gated according to their DNA fluorescence intensity distribution and the error bars represent SEM of ~600 G1 nuclei measured by LSC. The cell-to-cell and sample-to-sample C.V. values of the elution profiles are shown in Supplementary Table S1.