Table 1 Comparison of QINESIn with other methods available for the examination of molecular features related to nucleosome stability.
From: Nucleosome stability measured in situ by automated quantitative imaging
QINESIn | Proteomic analyses Refs (A) | Assays on isolated/reconstituted nucleosomes Refs (B) | Genomics approaches Refs (C) | Plasmid derived tagged histones Refs (D) | |
|---|---|---|---|---|---|
Quantitative analysis of nucleosome stability | + | + | + | + | + |
Histone PTM specificity | + | + | + − | + | − |
Histone variant specificity | + | + | + | + | + |
Overall expression or modification levels assessed | + | + | − | + − | − |
Measurement targets endogenous histones | + | + | + | + | − |
Measurement of nucleosome stability in situ | + | − | − | − | + |
Analyses according to cell cycle phases, without synchronization* | + | − | − | − | − |
Assessment of superhelicity effects in situ** | + | − | − | − | − |
Detection of molecular interactions by X-linking*** | + | − | − | − | + |
High-throughput screening | + | − | − | − | − |
Comparison of different cell types in mixed-cell experiments**** | + | − | − | − | − |
Analysis according to cell surface markers in mixed-cell experiments | + | − | − | − | − |
Cell-by-cell analyses | + | − | − | − | + |
Sensitivity to heterogeneity (e. g. gating for different expression levels)***** | + | − | − | − | + |
Picturing genome-wide distribution | − | − | − | + | − |
