Figure 5 | Scientific Reports

Figure 5

From: The transcriptional regulator NtrC controls glucose-6-phosphate dehydrogenase expression and polyhydroxybutyrate synthesis through NADPH availability in Herbaspirillum seropedicae

Figure 5

The ntrC mutant presents increased G6PDH activity and NADPH/NADP+ ratio. The G6PDH (A), GDH (B) and ME (C) activities were measured in crude extracts of the SmR1 (black bars) and ntrC (red bars) strains cultivated at 30 °C in NFbHP with 25 mM of glucose and 20 mM of ammonium chloride. (C) The transcriptional activity of the Ppgi-gfp fusion was measured during SmR1 (red circles) and ntrC mutant (blue circles) cultivation at 30 °C in NFbHP with 25 mM of glucose and 20 mM of ammonium chloride. The NADH/NAD+ (black bars) and NADPH/NADP+ (red bars) ratios were measured for strains SmR1 (E), ntrC (F) and both strains complemented with pKRT1. Where appropriate, statistical significance is shown (*−p-value ≤ 0.05, independent two-sample t-test).

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