Table 2 Bacterial strains and plasmids used in this work.

From: The transcriptional regulator NtrC controls glucose-6-phosphate dehydrogenase expression and polyhydroxybutyrate synthesis through NADPH availability in Herbaspirillum seropedicae

Strain or plasmid

Relevant characteristics

Reference/source

E. coli

Top10

Cloning host

Thermo Fischer Scientific

S17-1

Conjugational transfer of plasmids

45

H. seropedicae

SmR1

Parental strain, Nif+, SmR

46

ntrC (DCP286A)

SmR1 containing ntrC::Tn5-B20

23

glnB

SmR1 containing glnB::TcR

47

glnK

SmR1 with a chromosomal deletion of the glnK

47

glnD

SmR1 containing glnD::TcR

Unpublished result

amtB

SmR1 containing amtB::lacZ-KmR

47

Plasmids

pBBR1MCS-3

 

48

pLAFR3

Broad-host-range cloning vector, IncP1, TcR

29

pKRT1

23-kb fragment from H. seropedicae SmR1 harbouring the glnA, ntrB and ntrC genes

46

pEK07

pBBR1MCS-3 containing pgi upstream region (Ppgi) from H. seropedicae SmR1 cloned between XhoI and SpeI restriction sites

This work

BBa_I13504

Plasmid pSB1C3-derivative containing the gfpmut3b gene (BBa_E0040) with the strong rbs (BBa_B0034) and the double terminator (BBa_B0015).

Registry of Standard Biological Parts (partsregistry.org)

pEKGFP01

pBBR1MCS-3 containing pgi upstream region (Ppgi) from H. seropedicae SmR1 and gfpmut3b downstream.

This work

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