Figure 5 | Scientific Reports

Figure 5

From: AlphaScreen-based homogeneous assay using a pair of 25-residue artificial proteins for high-throughput analysis of non-native IgG

Figure 5

Relationship between luminescence intensity of AlphaScreen signals and particle size of non-native IgG. (A) Detection of acid-stressed IgG by the AF.2A1-AlphaScreen technique. The acid-stressed IgG was incubated at 4 °C for 0, 6, 24, and 72 h. Time after neutralization of the IgG solution is indicated. (B) Analysis of acid-stressed IgG by DLS in conditions described in (A). (C) Detection of acid-stressed IgG by the AF.2A1-AlphaScreen technique. The acid-stressed IgG was incubated at 25 °C and treated as in (A). (D) Analysis of acid-stressed IgG by DLS in conditions described in (C). Three independent experiments were performed, and the data were presented as mean ± SD. MAb_A was used in this experiment.

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