Figure 1
From: Schwann cells promote post-traumatic nerve inflammation and neuropathic pain through MHC class II
Characterization of the P0CreIAbfl/fl mouse line. (a) P0Cre mice were crossed with IAbfl/fl mice to generate homozygous P0CreIAbfl/fl mice. Wildtype IAbfl/fl mice served as controls in all experiments. Acknowledgements for drawings of mice to Prof. Dr. Sven Meuth and Heike Blum, Institute for Translational Neurology, University Hospital Münster, Münster, Germany. (b) One week after chronic constriction injury, longitudinal paraffin sections (thickness 6 µm) of the sciatic nerve from wildtype IAbfl/fl mice (top panels) and P0CreIAbfl/fl mice (bottom panels) were stained against S100 (green signal), MHC class II (MHC-II; red signal) using fluorescently labelled secondary antibodies; nuclei were stained with DAPI. White arrow indicates an MHC-II expressing macrophage. Right panels depict higher magnifications of the areas indicated in the left panels.
