Figure 4
From: Schwann cells promote post-traumatic nerve inflammation and neuropathic pain through MHC class II
Schwann cell–specific MHC class II deficiency reduces post-traumatic thermal hyperalgesia and mechanical allodynia in female mice. (a) Thermal hyperalgesia was tested 7 days after chronic constriction injury (CCI) by placing the mouse’s hind paw from the lesioned side onto a hot plate (temperature 40 °C) and recording the latency (in seconds) until paw withdrawal (see methods). The latency, averaged between three technical repeats, is depicted and every symbol indicates one mouse. Note that increased latency reflects decreased hyperalgesia. (b) The post-CCI latency was divided by the average pre-CCI latency in every mouse and is expressed as a proportion of pre-CCI latency. (c) Mechanical allodynia was measured with von Frey filaments 7 days after CCI by pressing von Frey filaments of increasing strength against the plantar surface of the hind paw on the lesioned side and recording the thickness of filaments that induced paw withdrawal reaction. The corresponding force (in grams) was calculated (see methods). Note that increased threshold reflects decreased allodynia. (d) The post-CCI force was divided by the average pre-CCI force and is expressed as a proportion of pre-CCI force. Data were compared using unpaired t-test with Welch’s correction. *p-value < 0.05, **p-value < 0.01. Pooled data from three (hot plate) and two (von Frey filaments) independent experiments, respectively, with in total ≥8 female mice per group (wildtype IAbfl/fl vs. P0CreIAbfl/fl mice), are depicted.
