Figure 3 | Scientific Reports

Figure 3

From: Evaluation of irreversible protein thermal inactivation caused by breakage of disulphide bonds using methanethiosulphonate

Figure 3The alternative text for this image may have been generated using AI.

Suppression of BLA disulphide bond breakage by MMTS. (A) Size-exclusion HPLC elution profiles for BLA (20 µg) upon heating at 100 °C in the presence or absence of 1 mM MMTS. (B) SDS-PAGE analysis of BLA migration changes upon heating with or without thiol reactive reagents. All heating experiments were performed in HEPES buffer, pH 6.8.

Back to article page