Figure 4 | Scientific Reports

Figure 4

From: Evaluation of irreversible protein thermal inactivation caused by breakage of disulphide bonds using methanethiosulphonate

Figure 4The alternative text for this image may have been generated using AI.

Effects of MTS reagents on IgG conformation upon heating. (A) Size-exclusion HPLC analysis of IgG1 (OKT9). The molecular sizes of IgG in HEPES buffer, pH 6.8, were analysed after heating at 60 °C or 70 °C for 2 h with or without 1 mM MMTS. (B) Disulphide-shuffled polymerization of heat-treated IgG was analysed by SDS-PAGE in the presence or absence of reducing agents.

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