Figure 3
Panel A displays hematoxylin-eosin eosin staining of bone and bone marrow biopsy in a sham mouse with magnification related to the green insert. Panel B displays the same analysis in an untreated xenograft model (relative to the red insert): a high number of multinucleated large cells similar to osteoclasts are evident (white and red arrows). Panel C displays the absence of this finding in the bone marrow of a mouse subjected to Denosumab treatment for three weeks (blue insert). Panel D reports the original images of the right femur obtained by high resolution CT in a control model and in a CLL mouse, respectively. The longitudinal sections are represented in the middle and connected with the corresponding short axis slices in the original CT scans obtained in living animal. The enlargement in intrabone section is apparent. Alongside these images, Panel E displays a 3D representation of high resolution CT obtained ex vivo from the same femur. These images document a relative loss in trabecular structure as well as an irregular border of compact bone. This pattern is confirmed in the original 2D sections of the same microCT scan (panel F) in which compact bone erosion of femur shaft is detailed (bottom inserts).
