Figure 5

Gross anatomy of non-peptidergic nociceptors in the spinal cord and skin is normal in TrpC3 null mice. (A and B) Immunostaining in P21 Mrgprd ^EGFP/+ and Mrgprd ^EGFP/+; TrpC3 ^−/− mouse thoracic spinal cord sections using antibodies against CGRP and GFP. (C and D) Immunostaining in P21 Mrgprd ^EGFP/+ and Mrgprd ^EGFP/+;TrpC3 ^−/− mouse glabrous skin cross sections using antibodies against CGRP and GFP. (E–J) Immunostaining in P21 Mrgprd ^EGFP/+ and Mrgprd ^EGFP/+;TrpC3 ^−/− mouse whole mount glabrous skin using antibodies against CGRP and GFP. No obvious differences are seen in spinal cord and peripheral nerve endings between WT and TrpC3 KO backgrounds. N = 3 mice for each genotype. Scale bars = 50 μm.