Figure 6

TRPC3 is not required for MRGPRD-evoked behavior or signaling at a cellular level. (A,B) Adult (>6 weeks old) TrpC3 KO and littermate WT control mice were scored for scratching behavior after β-alanine injection into the cheek and back. N = at least 7 mice per genotype for β-alanine back injection, at least 5 mice per genotype for β-alanine cheek injection. (C,D) Representative traces of calcium activity from littermate control and TrpC3 ^−/− null cultured DRG neurons. DRG neurons of both genotypes show normal responses to β-alanine, while activity in response to KCl demonstrates that the neurons are healthy and viable. (E–J) Representative images of DRG neurons from control (E–G) and TrpC3 ^−/− null (H–J) mice, with arrowheads indicating neurons that show responsiveness to both 1 mM β-alanine and 30 mM KCl. Percentages were pooled from results collected from 1,481 control and 990 TrpC3 KO DRG neurons from 6 animals per genotype. Scale bar = 50 μm. (K) Bar graph comparing the percentage of all DRG neurons that showed a positive response to β-alanine between littermate control and TrpC3 ^−/− null cultured DRG neurons. No significant difference was seen (9.9 ± 0.8% in control DRG neurons vs. 9.3 ± 1.2% in TrpC3 KO DRG neurons; student t test). (K) Adult (>6 weeks old) TrpC3 KO and littermate WT control mice were scored for latency to fall off rotarod apparatus. N = 9 mice per genotype.