Figure 3

Effect of 1 mM melatonin on morphological changes and lipid accumulation in BIPs. (A) Representative images of unstained or oil-red O stained cells after 8 days of differentiation; (B) the average size of 100 lipid droplets per sample was measured on Image J software; (C) effects of melatonin on lipid accumulation. Scale bar = 50 μm. Values are presented as the means ± S.E.M. “*”represents significant differences, P < 0.05; “**”represents significant differences P < 0.01. BIPs = bovine intramuscular preadipocytes.