Figure 5 | Scientific Reports

Figure 5

From: Melatonin promotes triacylglycerol accumulation via MT2 receptor during differentiation in bovine intramuscular preadipocytes

Figure 5

Effect of luzindole or 4P-PDOT on melatonin-induced ROS production in BIPs. (A) ROS staining (green fluorescence) in BIPs after differentiation for 8 days with or without melatonin, luzindole or 4P-PDOT; (B) effect of luzindole and 4P-PDOT on melatonin-induced ROS production; (C) the mRNA expression of antioxidant genes (SOD1 and GPX4); (D) the activities of antioxidant enzymes SOD1 and GPX4. The mRNA expression of antioxidant genes were normalized to housekeeping genes β-actin and GAPDH. Values are presented as the means ± S.E.M. “**”represents significant differences P < 0.01. BIPs = bovine intramuscular preadipocytes; 4P-PDOT = 4-phenyl-2-propionamidotetraline; SOD1 = superoxide dismutase 1; GPX4 glutathione peroxidase 4.

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