Figure 6 | Scientific Reports

Figure 6

From: Melatonin promotes triacylglycerol accumulation via MT2 receptor during differentiation in bovine intramuscular preadipocytes

Figure 6

Expression profiles of MT1 and MT2 at different stages of BIP differentiation. (A) The mRNA expression of the MT1 and MT2 genes at 3, 8 and 12 d of BIPs differentiation; (B) proteins level of MT1 and MT2 were quantitated using densitometry and normalized to β-actin levels; (C) Effect of melatonin on the mRNA and protein expression of the MT1 and MT2 genes at 8 d of BIPs differentiation. The expression of MT1 and MT2 mRNA were normalized to housekeeping genes β-actin and GAPDH. Protein level of MT1 and MT2 were quantitated using densitometry and normalized to β-actin levels. Values are presented as the means ± S.E.M. “**”represents significant differences P < 0.01. MT1 = melatonin receptors 1; MT2 = melatonin receptors 2; BIPs = bovine intramuscular preadipocytes.

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