Figure 2
From: Nkx2.5+ Cardiomyoblasts Contribute to Cardiomyogenesis in the Neonatal Heart
Phenotypic characterization of neonatal Nkx2.5 enh-eGFP + cells. (A) Analysis of surface marker expression in eGFP + and eGFP- non-myocytes from the neonatal Nkx2.5 enh-eGFP heart. Shown are representative flow plots (n = 4). (B) Quantification of the percentage of eGFP + and eGFP- cells that express the indicated surface marker. (C) Genome-wide expression analysis of embryonic day 13.5 (n = 10), 16.5 (n = 10), and neonatal P7 (n = 10) (total) GFP + cells against neonatal CMs (neo CM) (n = 10) and adult cardiac fibroblasts (n = 5). (D) Comparison of genome-wide transcriptional profile of neonatal eGFP + cells with embryonic day 10.5 (e10.5) CMs, isolated from wild type mice. (E,F) Quantitative PCR analysis of cardiac transcription factor (E) and sarcomeric gene (F) expression in neonatal day 7 (P7) eGFP + cells and e10.5 CMs.
