Figure 4
From: Nkx2.5+ Cardiomyoblasts Contribute to Cardiomyogenesis in the Neonatal Heart
Generation and characterization of doxycycline-regulated Nkx2.5 enhancer-Cre transgenic mice. (A) Diagram of the plasmid construct used to generate the transgenic mice (top panel). Bottom panel: confirmation of the presence of transgenic sequence in founder mice by genomic PCR analysis. (B) Whole-mount fluorescence microscopy of Nkx2.5 enh-Cre embryos at days 8.0 and 9.5 post coitum (n = 2). Note the cardiac-restricted expression of Cre-eGFP. (C) Analysis of Cre + cells and their lineage descendants in Nkx2.5 enh-Cre /ROSA26FSLacZ transgenic embryos (n = 3) at 12.5 day post coitum in the absence (a,b,c) or presence (d,e,f) of doxycycline administration. (a,d) Whole mount bright field microscopy of X-Gal stained embryos. (b,c,e,f) Transverse sections of X-Gal stained embryos at day 12.5 post-coitum. Mid-Vent: Mid-ventricular. OFT: outflow tract.
