Figure 8
Time-lapse imaging of miR-132-3p activation in living neurons. (a) Cortical neurons (DIV 5) were stimulated with 50 ng/mL BDNF for 12 h. Expression of miR-132-3p was analyzed by quantitative RT-PCR. Data are expressed as mean ± SD (n = 6). (b) pdsVenus-132-T/dsCFP vector. (c) Cortical neurons were transfected with pdsVenus-132-T/dsCFP. Fluorescence of dsVenus-132-T (left) and dsCFP (right) are shown. Scale bars: 20 µm. (d) Neurons were transfected with pdsVenus/dsCFP or pdsVenus-132-T/dsCFP and were analyzed by time-lapse imaging. Fluorescence of dsVenus and dsCFP were captured every 20 min at 37 °C. BDNF (50 ng/mL) was added at the indicated time. The dsVenus/dsCFP ratio are presented as mean ± SEM. Numbers of cells analyzed are shown in parentheses. *P < 0.05, **P < 0.01 (two-way repeated measures ANOVA).
