Table 2 Top 100 enriched gene disruptions beneficial to IMP expression identified.
From: Improving membrane protein expression and function using genomic edits
Rank | Gene | Number of enriched replicates in each libarary (TnLib/…) | Overall Number of Replicates | Observed in Number of Libraries | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
cyoB-GFP | cydB-GFP | lepI-GFP | mdlB-GFP | yidC-GFP | gstA-GFP | Total replicates | IMP-only replicates | Total frequency | IMP frequency | ||
1 | hflD | 5 | 2 | 2 | 0 | 2 | 0 | 11 | 11 | 4 | 4 |
2 | dadX | 5 | 1 | 1 | 2 | 1 | 1 | 11 | 10 | 6 | 5 |
3 | rep | 3 | 3 | 2 | 0 | 2 | 0 | 10 | 10 | 4 | 4 |
4 | ybeZ | 5 | 2 | 2 | 1 | 0 | 0 | 10 | 10 | 4 | 4 |
5 | yceD | 5 | 1 | 2 | 0 | 2 | 0 | 10 | 10 | 4 | 4 |
6 | astD | 5 | 0 | 2 | 0 | 2 | 1 | 10 | 9 | 4 | 3 |
7 | proQ | 5 | 0 | 2 | 0 | 2 | 0 | 9 | 9 | 3 | 3 |
8 | ydhZ | 5 | 1 | 2 | 0 | 1 | 0 | 9 | 9 | 4 | 4 |
9 | yeeW | 5 | 0 | 2 | 0 | 2 | 2 | 11 | 9 | 4 | 3 |
10 | aldA | 4 | 2 | 1 | 1 | 0 | 0 | 8 | 8 | 4 | 4 |
11 | tatA | 2 | 0 | 2 | 2 | 2 | 1 | 9 | 8 | 5 | 4 |
12 | cfa | 5 | 0 | 2 | 0 | 1 | 1 | 9 | 8 | 4 | 3 |
13 | edd | 5 | 0 | 2 | 0 | 1 | 2 | 10 | 8 | 4 | 3 |
14 | fabF | 5 | 0 | 2 | 0 | 1 | 0 | 8 | 8 | 3 | 3 |
15 | fliN | 5 | 0 | 2 | 0 | 1 | 0 | 8 | 8 | 3 | 3 |
16 | hspQ | 5 | 0 | 2 | 0 | 1 | 1 | 9 | 8 | 4 | 3 |
17 | nlpC | 3 | 2 | 2 | 1 | 0 | 0 | 8 | 8 | 4 | 4 |
18 | oppC | 5 | 0 | 2 | 0 | 1 | 0 | 8 | 8 | 3 | 3 |
19 | oppD | 5 | 0 | 2 | 0 | 1 | 0 | 8 | 8 | 3 | 3 |
20 | pspE | 4 | 1 | 2 | 0 | 1 | 0 | 8 | 8 | 4 | 4 |
21 | ybgE | 5 | 1 | 2 | 0 | 0 | 0 | 8 | 8 | 3 | 3 |
22 | yedE | 5 | 0 | 2 | 0 | 1 | 2 | 10 | 8 | 4 | 3 |
23 | yedI | 5 | 1 | 2 | 0 | 0 | 0 | 8 | 8 | 3 | 3 |
24 | astA | 5 | 0 | 2 | 0 | 0 | 2 | 9 | 7 | 3 | 2 |
25 | ccmA | 5 | 0 | 2 | 0 | 0 | 1 | 8 | 7 | 3 | 2 |
26 | cheZ | 5 | 1 | 1 | 0 | 0 | 0 | 7 | 7 | 3 | 3 |
27 | cysA | 5 | 0 | 2 | 0 | 0 | 0 | 7 | 7 | 2 | 2 |
28 | dinI | 5 | 0 | 1 | 1 | 0 | 2 | 9 | 7 | 4 | 3 |
29 | hpt | 2 | 3 | 2 | 0 | 0 | 0 | 7 | 7 | 3 | 3 |
30 | htpG | 5 | 0 | 2 | 0 | 0 | 1 | 8 | 7 | 3 | 2 |
31 | mhpT | 5 | 1 | 1 | 0 | 0 | 0 | 7 | 7 | 3 | 3 |
32 | minD | 0 | 3 | 1 | 1 | 2 | 1 | 8 | 7 | 5 | 4 |
33 | narJ | 5 | 1 | 1 | 0 | 0 | 1 | 8 | 7 | 4 | 3 |
34 | nfo | 5 | 0 | 2 | 0 | 0 | 1 | 8 | 7 | 3 | 2 |
35 | oppF | 5 | 0 | 2 | 0 | 0 | 1 | 8 | 7 | 3 | 2 |
36 | tyrR | 3 | 1 | 2 | 0 | 1 | 0 | 7 | 7 | 4 | 4 |
37 | uidC | 4 | 1 | 2 | 0 | 0 | 0 | 7 | 7 | 3 | 3 |
38 | ybaZ | 4 | 0 | 2 | 1 | 0 | 0 | 7 | 7 | 3 | 3 |
39 | ycfJ | 5 | 0 | 2 | 0 | 0 | 1 | 8 | 7 | 3 | 2 |
40 | ydcW | 5 | 0 | 2 | 0 | 0 | 1 | 8 | 7 | 3 | 2 |
41 | ydjB | 4 | 0 | 2 | 0 | 1 | 0 | 7 | 7 | 3 | 3 |
42 | yebC | 5 | 0 | 2 | 0 | 0 | 2 | 9 | 7 | 3 | 2 |
43 | yedF | 5 | 0 | 0 | 0 | 2 | 2 | 9 | 7 | 3 | 2 |
44 | ydfT | 5 | 0 | 0 | 1 | 0 | 0 | 6 | 6 | 2 | 2 |
45 | cchB | 4 | 1 | 1 | 0 | 0 | 0 | 6 | 6 | 3 | 3 |
46 | chaA | 2 | 3 | 1 | 0 | 0 | 0 | 6 | 6 | 3 | 3 |
47 | cyaA | 1 | 3 | 2 | 0 | 0 | 0 | 6 | 6 | 3 | 3 |
48 | eutL | 3 | 1 | 2 | 0 | 0 | 0 | 6 | 6 | 3 | 3 |
49 | fadR | 2 | 0 | 2 | 0 | 2 | 0 | 6 | 6 | 3 | 3 |
50 | hybF | 3 | 1 | 2 | 0 | 0 | 0 | 6 | 6 | 3 | 3 |
51 | ilvL | 5 | 0 | 1 | 0 | 0 | 0 | 6 | 6 | 2 | 2 |
52 | napG | 4 | 0 | 2 | 0 | 0 | 0 | 6 | 6 | 2 | 2 |
53 | narH | 5 | 0 | 1 | 0 | 0 | 0 | 6 | 6 | 2 | 2 |
54 | nuoG | 5 | 0 | 1 | 0 | 0 | 0 | 6 | 6 | 2 | 2 |
55 | otsB | 2 | 0 | 2 | 1 | 1 | 0 | 6 | 6 | 4 | 4 |
56 | purR | 5 | 0 | 1 | 0 | 0 | 0 | 6 | 6 | 2 | 2 |
57 | rseA | 5 | 0 | 1 | 0 | 0 | 1 | 7 | 6 | 3 | 2 |
58 | trxA | 2 | 3 | 0 | 0 | 1 | 0 | 6 | 6 | 3 | 3 |
59 | wzzB | 3 | 0 | 1 | 0 | 2 | 0 | 6 | 6 | 3 | 3 |
60 | yaaX | 3 | 1 | 2 | 0 | 0 | 0 | 6 | 6 | 3 | 3 |
61 | ybhA | 5 | 0 | 1 | 0 | 0 | 0 | 6 | 6 | 2 | 2 |
62 | yddE | 4 | 0 | 2 | 0 | 0 | 0 | 6 | 6 | 2 | 2 |
63 | yeiH | 4 | 0 | 2 | 0 | 0 | 1 | 7 | 6 | 3 | 2 |
64 | yfgL | 3 | 0 | 2 | 0 | 1 | 0 | 6 | 6 | 3 | 3 |
65 | yjgA | 5 | 0 | 1 | 0 | 0 | 1 | 7 | 6 | 3 | 2 |
66 | yqjH | 5 | 0 | 1 | 0 | 0 | 0 | 6 | 6 | 2 | 2 |
67 | znuB | 5 | 0 | 0 | 0 | 1 | 0 | 6 | 6 | 2 | 2 |
68 | argK | 4 | 0 | 1 | 0 | 0 | 0 | 5 | 5 | 2 | 2 |
69 | aroD | 0 | 2 | 1 | 2 | 0 | 0 | 5 | 5 | 3 | 3 |
70 | astC | 3 | 0 | 1 | 0 | 1 | 2 | 7 | 5 | 4 | 3 |
71 | citE | 2 | 1 | 2 | 0 | 0 | 1 | 6 | 5 | 4 | 3 |
72 | hrpA | 1 | 3 | 1 | 0 | 0 | 0 | 5 | 5 | 3 | 3 |
73 | hycA | 4 | 0 | 1 | 0 | 0 | 0 | 5 | 5 | 2 | 2 |
74 | hycF | 4 | 0 | 1 | 0 | 0 | 0 | 5 | 5 | 2 | 2 |
75 | hycG | 3 | 0 | 2 | 0 | 0 | 0 | 5 | 5 | 2 | 2 |
76 | hyfI | 4 | 0 | 1 | 0 | 0 | 1 | 6 | 5 | 3 | 2 |
77 | narK | 4 | 0 | 1 | 0 | 0 | 1 | 6 | 5 | 3 | 2 |
78 | narY | 2 | 0 | 2 | 1 | 0 | 0 | 5 | 5 | 3 | 3 |
79 | nuoN | 4 | 0 | 1 | 0 | 0 | 0 | 5 | 5 | 2 | 2 |
80 | oppA | 3 | 0 | 2 | 0 | 0 | 0 | 5 | 5 | 2 | 2 |
81 | paaH | 3 | 0 | 2 | 0 | 0 | 1 | 6 | 5 | 3 | 2 |
82 | psiF | 3 | 1 | 1 | 0 | 0 | 0 | 5 | 5 | 3 | 3 |
83 | rne | 0 | 3 | 2 | 0 | 0 | 0 | 5 | 5 | 2 | 2 |
84 | soxS | 2 | 1 | 2 | 0 | 0 | 0 | 5 | 5 | 3 | 3 |
85 | tig | 5 | 0 | 0 | 0 | 0 | 0 | 5 | 5 | 1 | 1 |
86 | trg | 2 | 1 | 1 | 1 | 0 | 0 | 5 | 5 | 4 | 4 |
87 | tyrP | 4 | 0 | 0 | 1 | 0 | 0 | 5 | 5 | 2 | 2 |
88 | usg | 5 | 0 | 0 | 0 | 0 | 1 | 6 | 5 | 2 | 1 |
89 | yagF | 5 | 0 | 0 | 0 | 0 | 0 | 5 | 5 | 1 | 1 |
90 | yajI | 3 | 1 | 1 | 0 | 0 | 1 | 6 | 5 | 4 | 3 |
91 | ybfF | 4 | 0 | 1 | 0 | 0 | 1 | 6 | 5 | 3 | 2 |
92 | ybiB | 4 | 0 | 1 | 0 | 0 | 0 | 5 | 5 | 2 | 2 |
93 | ycbC | 0 | 3 | 2 | 0 | 0 | 2 | 7 | 5 | 3 | 2 |
94 | ycdZ | 4 | 0 | 1 | 0 | 0 | 0 | 5 | 5 | 2 | 2 |
95 | ycfD | 4 | 0 | 1 | 0 | 0 | 1 | 6 | 5 | 3 | 2 |
96 | ydhF | 4 | 0 | 0 | 1 | 0 | 0 | 5 | 5 | 2 | 2 |
97 | ydhP | 2 | 0 | 1 | 0 | 2 | 0 | 5 | 5 | 3 | 3 |
98 | yebS | 3 | 0 | 2 | 0 | 0 | 1 | 6 | 5 | 3 | 2 |
99 | yebZ | 3 | 0 | 2 | 0 | 0 | 1 | 6 | 5 | 3 | 2 |
100 | yegW | 3 | 0 | 2 | 0 | 0 | 0 | 5 | 5 | 2 | 2 |
