Figure 2

Dystrophin isoforms expression across brain development. (A) Dystrophin exons expression throughout brain development. The isoform unique first exons are indicated on top of the heatmap. The developmental stages are indicated on the left in post-conceptual weeks and months or years after birth. The BrainSpan atlas exon number is indicated below the heatmap together with the Dp427m exon numbering for reference. Bars below the heatmap indicate the different isoform groups. The grey bars corresponding to Dp427c,m,p are grouped together using exon 2 in further analysis (blue). The grey bars corresponding to Dp260 and Dp116 are expressed in the retina and peripheral nerves and are excluded from further analysis. The first exon of Dp140 (green) and Dp71 + Dp40 (orange) is used for further analysis. Boxes with a red ‘X’ indicate exons that are not part of the transcript. Expression values are presented as log₂(RPKM). Brain region specific expression across development is shown for the cerebral cortex (B), hippocampus (C), amygdala (D) and cerebellum (E) of Dp427 (2^nd exon), Dp140 (1^st exon) and Dp71 + Dp40 (1^st exon). PCW: post-conception week, Mon: months, Yrs: years.