Figure 1

Oncogenic vs wild-type KRAS influences the role of NRP1 in tumorigenesis in pancreatic and lung cancers. (A) Western blot analysis of NRP1 knockdown levels in PDAC and NSCLC cells. (B–E) Cell viability for different PDAC cells BxPC-3 (B) and PANC-1 (C) and NSCLC cells H226 (D) and A549 (E) with reduced NRP1 expression grown in 3D culture for 72 hours. Data are plotted as percentage of control cells (transfected control shRNA -cont sh). All data are presented as the mean ± SD of 3 independent experiments. *P < 0.05 vs control; **P < 0.01 vs control.