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Figure 1

From: Neofunctionalization of “Juvenile Hormone Esterase Duplication” in Drosophila as an odorant-degrading enzyme towards food odorants

Figure 1The alternative text for this image may have been generated using AI.

Jhedup expression in antennae. (a) Normalized expression of jhedup and jhe in antennae + maxillary palps, proboscises, heads without appendages, legs and bodies of wildtype (Canton-S) males and females using qPCR (reference gene for normalization of expression: pgk). The normalized expression level is indicated as mean ± SE of triplicate biological samples. (b) Expression of jhedup in antennae and maxillary palps of jhedup-GAL4; UAS-mCD8::GFP flies. Scale bar, 150 µm. (c) jhedup expression in the 3rd antennal segment of jhedup-GAL4; UAS-mCD8::GFP flies (anterior and posterior view), including scheme for dispersion of large basiconics on the 3rd antennal segment, scale bar 20 µm. (d) Antennal slice of 3rd antennal segment. Scale bar, 20 µm. (e) Jhedup expression in 3rd antennal segment of jhedup-GAL4; UAS-mCD8::Cherry//elav-LexA; LexAOP-mCD8::GFP flies. Jhedup expression (red), expression of neuronal marker elav (green). (f) Higher magnification of (e), Scale bar, 3 µm. Jhedup expression in 3rd antennal segment of (g) jhedup-GAL4; UAS-mCD8::Cherry//OR42b::GFP and (h) jhedup-GAL4; UAS-mCD8::Cherry//OR22a::GFP flies. Jhedup expression (red), expression of olfactory receptor OR42b (ab1A neuron) or OR22a (ab3A neuron) coupled to GFP (green), detailed view. Scale bars (f/g), 6 µm.

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