Figure 1
MDSCs are induced after TxT. Sham- or TxT-treated mice were analysed at different time points after TxT for the induction of MDSCs by staining splenocytes and lung leukocytes with Gr-1 and CD11b. (a) Absolute numbers of MDSCs were calculated in the spleen and in the lung. Data present the mean value ± SD for the following numbers of mice analysed: 6 h:n = 11; 18 h:n = 8; 24 h:n = 11; 48 h:n = 11; 72 h:n = 15; 5d:n = 15; *P ≤ 0.05; **P ≤ 0.01. Significance was calculated by Student’s t test comparing sham and TxT mice at each time point. (b) Mice received TxT or were sham-treated and 24 hours later lung cells were stained for CD11b, Gr-1, CD11c. Cells were gated by Gr-1 expression levels (P1 (red), P2 (black), P3 (green)). Representative flow data of one mouse out of three mice analysed are depicted and numbers represent the percentage of cells in P1, P2 and P3. Cells in P1, P2, P3 were analysed for CD11c expression. (c) Isolated lung cells of sham- and TxT-treated animals were stained for CD11b, CD11c, Ly-6G and Ly-6C and CD11b+CD11c- cells were analysed for the expression of Ly-6C and Ly-6G. Representative flow data of one mouse out of three mice analysed are shown.
