Figure 5
From: Cibinetide dampens innate immune cell functions thus ameliorating the course of experimental colitis
Cibinetide inhibits pro-inflammatory immune responses in BMDMs in vitro via CD131. BMDMs differentiated from CD131 −/− mice or C57BL/6 N mice were pre-treated with PBS as solvent (Ctrl.), 2 pmol/mL rhu erythropoietin (EPO), 2 pmol/mL cibinetide (CIB) or 50 µg/ml dexamethasone (DEX) 30 min before the addition of LPS (200 ng/mL) or solvent. Supernatants were analyzed for concentrations of cytokines after 24 hours. Data from 4 independent experiments were compared by means of ANOVA using Bonferroni correction for multiple testing. Values are depicted as means ± S.E.M. and statistical significances between PBS and EPO, cibinetide or dexamethasone treatment, respectively, are indicated. Ccl2 (A), TNF (B), IL-1ß (C) and IL-6 (D) levels in supernatants are depicted. IL-1ß and IL-6 levels of solvent-treated control macrophages remained below the reported detection limits of the corresponding ELISA kits. *p < 0.05 as compared to LPS/solvent stimulation in the respective genotype group; n.d. denotes not detectable.
