Figure 2 | Scientific Reports

Figure 2

From: Performance and consistency of a fluorescence-based high-throughput screening assay for use in Babesia drug screening in mice

Figure 2

Optimization of the best condition of SYBR Green I (SG I) staining for fluorescence assay in mice using different concentrations of the stain. (a) 0.25x SG I; (b) 0.5x SG I; (c) 1x SG I; (d) 2x SG I; (e) 4x SG I; (f) 8x SG I. Each value represents the mean of duplicate trails of 5 mice per experimental group after subtracting the background fluorescence of non-parasitized RBCs from uninfected mice. Gain values are not set to 100.

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