Figure 3 | Scientific Reports

Figure 3

From: Performance and consistency of a fluorescence-based high-throughput screening assay for use in Babesia drug screening in mice

Figure 3

Comparison of the emitted fluorescence signals and parasitemia detected by fluorescence- and microscopy-based methods, respectively, using different concentrations of SYBR Green I stain. (a) Microscopy-based method; (b) fluorescence-based method using 1x SG I stain; (c) fluorescence-based method using 2x SG I stain; (d) fluorescence-based method using 4x SG I stain; (e) fluorescence-based method using 8x SG I stain. Each value represents the mean ± standard deviation of 5 mice per experimental group after subtracting the background fluorescence for non-parasitized RBCs from uninfected mice. Gain values are not set to 100.

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