Figure 5 | Scientific Reports

Figure 5

From: Performance and consistency of a fluorescence-based high-throughput screening assay for use in Babesia drug screening in mice

Figure 5

Comparison of the inhibitory effects of selected antibabesial drugs on the growth of Babesia microti estimated by microscopy- and fluorescence-based methods in uninfected and infected mice. (a) Inhibitory effect of diminazene aceturate (DA) estimated by fluorescence-based method; (b) inhibitory effect of diminazene aceturate (DA) estimated by microscopy-based method; (c) inhibitory effect of pyronaridine tetraphosphate (PYR) estimated by fluorescence-based method; (d) inhibitory effect of pyronaridine tetraphosphate (PYR) estimated by microscopy-based method; (e) inhibitory effect of allicin/DA combination estimated by fluorescence-based method; (f) inhibitory effect of allicin/DA combination estimated by microscopy-based method. Each value represents the mean ± standard deviation of 5 mice per experimental group after subtracting the background fluorescence for non-parasitized RBCs from uninfected mice. Asterisks indicate significant differences (*P < 0.05) between treated and control mice. Student’s t-test, P < 0.05 between control and treated groups by fluorescence- and microscopy-based methods. Gain values are not set to 100.

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