Table 1 Statistical parameters for determining the quality of high-throughput screening (HTS) assay in Babesia microti parasite with different HCTs.

From: Performance and consistency of a fluorescence-based high-throughput screening assay for use in Babesia drug screening in mice

Parameters

Day 5

Day 6

Day 7

HCTs %

 

2.5

5

2.5

5

2.5

5

Z′ factor

0.89

−0.16

0.74

0.35

0.84

0.13

S/N ratio

122.41

63.51

230.97

117.67

570.99

39.56

% CVmax

2.53

33.31

8.28

490.57

4.91

22.99

% CVmin

8.17

13.74

36.97

24.54

3.01

15.99

  1. S/N ratio = Signal-to-noise ratio.
  2. % CVmax = the coefficient of variation at the maximum signal.
  3. % CVmin = the coefficient of variation at the minimum signal.