Figure 8
Dynamics of UPIb/UPIIIa-EGFP positive vesicles in non-treated, NOC and CytD treated MDCK cells. Movement of individual UPIb/UPIIIa-EGFP positive vesicles was monitored by time-lapse confocal microscopy in MDCK cells with synchronized protein release from the GA (A–C). Individual UPIb/UPIIIa-EGFP positive vesicles were tracked by software ParticleTR. The movement of UPIb/UPIIIa-EGFP positive vesicles and their trajectories are shown. (A) In non-treated UPIb/UPIIIa-EGFP expressing MDCK cells the UPIb/UPIIIa-EGFP transport proceeds normally from the GA to the PM. (B) The analysis shows that UPIb/UPIIIa-EGFP positive vesicles stop moving when the MTs are depolymerized. (C) The transport of UPIb/UPIIIa-EGFP is reduced, but not completely stopped when AFs are depolymerized. Bars: 10 µm (A-C).
