Figure 9
BN-PAGE analyses of thylakoid membrane protein complexes from Chuanmai42 and Sy95-71 under osmotic stress. Separation was achieved by BN-PAGE using 5-12.5% acrylamide. Thylakoid membranes (20 μg of Chl) solubilized using 1% (w/v) DM were subjected to BN-PAGE (A). PS, photosystem; NDH, NAD(P)H dehydrogenase; mc, megacomplex; sc, supercomplex; LHC, light-harvesting complex; Cyt b 6 /f, cytochrome b 6 /f. The bands of BN-PAGE were confirmed by immunoblotting with D1, Lhcb1, and PsaD specific antibodies (on the right). The control line of the BN gel was selected for immunodetection. (C) Quantitative analyses of LCHII assemblies and the PSII-LHCII supercomplexes under osmotic stress. Relative intensities of bands with respect to the content of 0 h (100%) are presented. Significant differences are presented with the asterisk at P < 0.05 level (Duncan’s multiplication range test). Values show the means ± SD (n = 4). 0–72 h represents osmotic stress for 0 h (control), 24 h, and 72 h.
