Figure 6
From: De novo mutations of TUBA3D are associated with keratoconus
ROS and SOD1, SOD2 expression levels of the wildtype, c.31 C > T (Gln11stop), and c.201insTT (Val68Leufs*2) mutation cells. HTK cells transiently transfected with constructs encoding HA-tagged wildtype and mutant (c.31 C > T, c.201insTT) TUBA3D proteins. (A) The cell morphology after 72 hours of transfection (B) ROS levels in HTK cells after 48 hours of transfection. n = 3. *P < 0.05. (C) SOD1 and SOD2 expression analysis in HTK cells after transfection by Western blotting. Representative figures of Western blotting (left, cropped from different gels of the same samples) and the relative protein content of each group (n = 3) quantified by Image J software (right). *P < 0.05. (D) The cell survival analysis of the HTK cells after 24, 48, 72 hours of transfection with (right) and without NAC (left).
