Figure 2

X-ray ptychography images of a live fission yeast zygote. (a) Successive image sequence of a live fission yeast zygote obtained by ptychographic CDI scans. (b) Phase shift histograms (plot of the number of pixels with a specific phase shift) of a zygote in the image without autophagic vacuoles (a, iii) and with autophagic vacuoles (a, vi). A positive shift corresponds to a lighter color. (c) Projected cell area and projected vacuole area versus the radiation dose calculated from the ptychographic zygote images. Each set of grey circles connected by a line corresponds to a different vacuole and the filled circles correspond to the mean area of the vacuoles. A characteristic dose of the onset of vacuole formation and a lethal dose can be identified (red dashed lines).